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3DCultr Intestinal Organoid Growth Medium (Mouse) is a serum-free medium that can be used for the establishment and long-term culture of mouse intestinal organoids derived from mouse intestinal stem cells (such as intestinal crypts). In the presence of extracellular matrix, the medium contains specific components and abundant growth factors that promote the rapid growth and formation of intestinal organoids from intestinal stem cells. The process of organoid formation is smooth and rapid, while maintaining high characteristics and vitality of intestinal stem cells. It supports subsequent research on intestinal physiological functions, diseases, and precision medicine based on organoids.

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3DCultr Intestinal Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of intestinal cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of intestinal cancer organoids from intestinal cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of intestinal cancer cells, which provides a basis for subsequent Provide support for physiological functions, disease research and precision medicine of intestinal cancer organoids.

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3DCultr Gastric Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of gastric cancer organoids derived from cells or tissues. In the presence of extracellular matrix, The unique components and abundant cytokines contained in the culture medium can promote the rapid growth and formation of gastric cancer organoids from gastric cancer cells. The organoid formation process is smooth and rapid, while maintaining high characteristics and vitality of gastric cancer cells, which provides a basis for subsequent support for physiological functions, disease research and precision medicine of gastric cancer organoids.

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3DCultr Liver Cancer Organoid Growth Medium (Human) is a serum-free medium that can be used for the establishment and long-term culture of liver cancer organoids derived from cells or tissues. Under the conditions of extracellular matrix, this medium contains unique components and abundant cytokines that promote the rapid growth and formation of liver cancer cells into liver cancer organoids.The process of organoid formation is smooth and rapid while maintaining the high characteristics and vitality of liver cancer cells, thereby providing support for subsequent studies on the physiological functions, disease research, and precision medicine based on liver cancer organoids.

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0,168 BITCOIN

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50000 SATOSHIS ZU BITCOIN

DfCell Mycoplasma Off Spray effectively inhibits the growth and contamination of fungi (including spores), bacteria (including spores), mycoplasma, and viruses (including HIV and Hepatitis B). Its active ingredient is a quaternary ammonium derivative. It is free from mercury, formaldehyde, phenol, alcohol, and other irritants, non-toxic, and biodegradable. It does not cause any damage to general work surfaces and equipment.

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DfCell Mycoplasma RT-qPCR Detection Kit is a rapid qualitative test based on Nucleic Acid Amplification Techniques (NAT) designed to detect potential Mycoplasma contamination in raw materials, cell banks, virus seeds, virus or cell harvests, and therapeutic cell products. This assay kit utilizes quantitative PCR technology employing a multiplex PCR approach with two fluorescent probes, FAM and VIC, to respectively detect the target sequence and internal reference. It covers over 100 Mycoplasma DNA sequences and has undergone rigorous validation for specificity, detection limit, and durability according to EP 2.6.7 standards. The detection limit meets the requirement of ≤10 CFU/mL, demonstrating high sensitivity, excellent specificity, and safety.

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The DfCell Mycoplasma LAMP Detection Kit can detect multiple types of mycoplasma, including the 8 common mycoplasma strains commonly found in cell culture. Traditional nested PCR mycoplasma detection methods are prone to false negative results due to the presence of inhibitors in the cell culture supernatant and require opening the lid for electrophoresis after the reaction, increasing the risk of false positive results due to contamination. The DfCell LAMP Kit completely eliminates these drawbacks and has high sensitivity and accuracy. 

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Dextran sulfate sodium(DSS) is a polyanionic derivative of dextran, formed by the esterification reaction of dextran and chlorosulfonic acid. It is commonly used in colitis modeling research. DSS has several characteristics:1) It is a polyanionic compound soluble in water, forming a colorless aqueous solution; 2) It exhibits high purity and good stability; 3) It is naturally degradable.

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D-Luciferin is a commonly used substrate for luciferase in the field of biotechnology, particularly in in vivo live imaging techniques. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice. Subsequently, D-luciferin is injected, and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or therapeutic efficacy of drugs in real-time. Alternatively, the impact of ATP on this reaction system can be utilized to indicate changes in energy or vital signs based on variations in bioluminescence intensity.

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D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs.

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Streptozocin (STZ) is an antibiotic produced by Streptomyces Aureobasidium pullulans. It has anti-tumor effects and is often used to treat pancreatic cancer. At the same time, STZ can selectively destroy pancreatic beta cells in certain species of animals and induce diabetes. It can be used for modeling research on diabetes models. Rats and mice are generally used to create animal models.

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The kit used for quantitative determination of FGF-10 in serum, plasma, and cell culture supernatants.

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The kit is used for the quantitative determination of mouse interleukin-6 (Mouse IL-6) in serum and plasma. Specific antibodies against mouse interleukin-6 are pre-coated on a high-affinity enzyme immunoassay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, the mouse interleukin-6 present in the samples binds to the solid-phase antibody.

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The kit is designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies.

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The kit is used for the quantitative measurement of IL-10 in serum, plasma, and cell culture supernatants. Specific anti-IL-10 antibodies are pre-coated on a high-affinity enzyme-linked immunosorbent assay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, IL-10 present in the samples binds to the solid-phase antibody.